Coding

Part:BBa_K3903000

Designed by: Maia Beeson, Julia Ashley & Aj Ridley   Group: iGEM21_St_Andrews   (2021-08-04)


Nonribosomal Peptide Synthetase (NRPS) (codon optimised for E.coli & fit for manufacture)

This part is an updated CDS of codon optimised nonribosomal peptide synthetase (NRPS) from our phase 1 project Part:BBa_K3634003, which now has a slightly changed sequence to accommodate manufacturer (IDT) specifications. NRPS is the final constitutive gene in the Shinogen cluster which converts sedoheptulose 7-phosphate to Shinorine. The product Shinorine has UV protectant properties as categorised by the Minnesota 2012 iGEM team (BBa_K814003).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 458
    Illegal BamHI site found at 2511
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None